Applications

QuantaMaster™ UV VIS (click for more)

QuantaMaster™ Luminescence (click for more)

QuantaMaster™ NIR (click for more)

  • Millisecond kinetics
  • DNA binding
  • Intracellular ion concentrations
    (Ca++, pH, etc.)
  • Phosphorescence
  • Membrane potential
  • FRET
  • Polarization/Anisotropy
  • Stopped Flow
  • Chemi-and bioluminescence
  • Fluorescence kinetics
  • Fluorescence ratios
  • Excitation ratios
  • Dynamic anisotropy
  • Steady state
  • Fluorescence
  • Solid fluorescence
  • Temperature dependant fluorescence
  • Intracellular Ion Determination
    (Ca++, pH, etc…)
  • LRET
  • Intrinsic Fluorescence of Proteins
  • Photoluminescence of OLED devices
  • Thin Films
  • Single Photon Counting
  • Analog Detection
  • Quantum Yield studies of solids and solutions
  • 4 Position peltier for higher throughput when doing long protein folding studies.
  • Impurity detection
  • Cell suspension work
  • More...
  • Protein folding
  • Phosphoresnce Lifetimes
  • Fluorescence suppression
  • Lanthanide emission and lifetimes
  • Solid-state phosphorescence
  • Delayed fluorescence
  • Time resolved spectra
  • Gated emission spectra
  • Gated excitation spectra
  • Microseconds to seconds kinetics
  • DNA binding
  • Intracellular ion concentrations
    (Ca++, pH, etc...)
  • Spectra and lifetimes of phosphors and inorganic materials
  • Membrane potential
  • FRET
  • LRET
  • Stopped Flow
  • Chemi-and bioluminescence
  • More...

 

  • Photochemistry
    Singlet oxtgen is frequent byproduct
  • Geology
    NIR luminescence of minerals
  • Forensic science
    Identifying forged documents
  • Photobiology and photomedicine
    Singlet oxygen detection (1270 nm)
  • Cancer treatment
    Photodynamic therapy (PDT)
  • Photobiology
    Photodegradation caused by singlet oxygen
  • Photosensitized oxidations
    Photooxidation of environmental pollutants
  • Optical fiber communication
    Optical amplifiers
    (e.g. chelated Er++ , 1540 nm)
  • Agriculture
    Development of environmentally friendly pesticides
  • More...
     

EasyRatioPro (click for more)

RatioMaster™ (click for more)

LaserStrobe™ (click for more)

Calcium Measurement

  • Intracellular free Ca2+
  • Mitochondrial Ca2+
  • Endoplasmic reticulum (ER) Ca2+ pool
  • Lysosomal Ca2+
  • Extracellular near membrane Ca2+

Membrane Potential

  • Plasma membrane Potential
  • Mitochondrial Membrane Potential

GFP & Variants

  • Identifying cells coexpressing GFP for analysis
  • Use mitoGFP to visualize mitochondria and measure mitochondrial calcium [Ca2+]m
  • Use ratiometric GFP (redox-GFP) to measure cellular/mitochondria reduce/oxidize reaction
  • HEK-293 cells transfected with the CaSR-pEGFP vector
  • ECFP
  • EYFP

Other Measurements

  • Calcium imaging of Single HEK 293 cell
  • Taste Bud
  • Pea Pollen tubes
  • Respiratory burst & reactive oxygen species (ROS)
  • NAD(P)H
  • Changes in CaM availability
  • Demonstration of selective plasma membrane permeabilization by digitonin in HEK293 cells
  • Detection by immunofluorescence of PfCRT expression in HEK293 cells
  • Receptor-mediated Glutamate release
    (by following NADH signal)
  • Fura-dextra
  • Cellular GSH level
  • FL-aldosterone efflux
  • Latency of response to exogenous ATP or ADP
  • Membrane traffic
  • Liposome
  • ER membrane
  • Cell contractile activity & cell Size
  • Cell Volume Measurement
  • Phagocytosis
  • Cell injury or death by PI fluorescent
  • Metal induced cytotoxity

Other Ions

  • Intracellular Mg2+
  • Intracellular Mn2+
  • Intracellular Zn2+
  • Intracellular Sr2+
  • Intracellular Na+
  • Intracellular Cl-
  • Cytosolic labile iron pool (LIP)
  • Iron homeostasis

pH

  • Intracellular pH
  • Vacuolar pH
  • Lysosomal pH

FRET

  • Donor Excitation Spectroscopy
  • Emission intensity
  • Donor emission intensity
  • Acceptor emission intensity
  • Ratio of donor/acceptor emission intensity
  • Measure molecular proximity
  • Binding of enzyme to itssubstrate
  • Measure Endoplasmic reticulum (ER) Ca2+

Calcium-related Measurement

  • Ca2+ Waves
  • Ca2+ wave quantitation
  • Ca2+ wave induced bymechanical stimulation
  • Ca2+ wave induced by mechanical wounding
  • Ca2+ wave propagation
  • In non-contacting cells
  • Over a physical gap
  • Ca2+ Oscillation induced by:
    • Hg2+
    • K+
    • Heat
    • ATP
    • Dopamine
    • FSH
    • Caffine
    • Ca2+ mobilization
    • Ca2+ homeostasis
    • Propagated cytoplasmic Ca2+cyt responses along the DVR wall.
  • Simultaneous Measurement of intracellular Ca2+ and:
    • Cell volume
    • Phygocytosis
    • Cl-
    • Oxidase activation
    • Ca2+-CaM
  • More...

 

Excitation-Shifted

Application

Dye

Calcium

Fura-2,
Fura PE3
FFP18 Quin-2
Fura-Red
Sodium SBFI
BCECF
HPTS
SNAFL®
DM-NERF®
CI-NERF®
Potassium PBFI
Magnesium Mag-Fura-2
Mag-Fura-5
Membrane Potential Di-4-ANEPPS
Di-8-ANEPPS

Non-Ratiometric

Application

Dye

Calcium




Fluo-3
Calcium Green™
Calcium Orange™
Calcium Crimson™
Rhod-2

Chloride

SPQ SPA
MQAE


HIV protease

HIV protein substrate

F-actin

phallotoxins

PKC

Fim-1
Rim-1

Emission-Shifted

Application

Dye

Calcium

Indo-1
pH SNARF-1®
SNARF-2®
Magnesium Mag-Indo-1
Membrane Fluidity Pyrene Fas
PATMAN
Free Fatty Acids ADIFABTM
Membrane Potential JC-1
Lipids, phoholipids Nile Red
Nucleic acids

Acridine
Orange

Dual Dye

Application

Dye

Nucleic acids

DAPI and FITC
FITC and APC
Fura Red™ and BCECF
FITC and Texas Red®
DAPI and PE

Common FRET Pairs

EGFP/DSRed2 FRET
CFP/DsRed2 FRET
BFP/DsRed2 FRET
CoralHue M-Cyan/K-Orange
GFP/Rhod2
FITC/CY3 FRET
FITC/TRITC FRET
YFP/TRITC FRET
CY3/CY5 FRET
CFP/YEP FRET
BFP/GFP FRET

And Many More!

 

Applications

Calcium-related Measurement

  • Ca2+ transients
  • Ca2+ mobilization
  • Ca2+ homeostasis
  • Ca2+ waves
  • Ca2+ oscillation
  • Ca2+ spikes
  • Ca2+ entry
  • Capacitative Calcium Entry (CCE)

Other Ions

  • Intracellular Mg2+
  • Intracellular Mn2+
  • Intracellular Zn2+
  • Intracellular Sr2+
  • Intracellular Na+
  • Intracellular Cl-
  • Intracellular Ba2+
  • Divalent cation influx
  • Labile iron pool (LIP)
  • Iron homeostasis
  • Na+/H+ exchange
  • H+/HCO3- exchange
  • Pf and PCO2

FRET

  • Measure molecular proximity
  • Binding of enzyme to its substrate
  • Measure Endoplasmic reticulum (ER) Ca2+
  • Donor Excitation Spectroscopy
  • Emission intensity
  • Donor emission intensity
  • Acceptor emission intensity
  • Ratio of donor/acceptor emission intensity

Other Measurements

  • Respiratory burst & reactive oxygen species (ROS)
  • NAD(P)H
  • Nitric Oxide (NO)
  • Intracellular GSH
  • Identifying cells coexpressing GFP for analysis
  • Use ratiometric GFP (redox-GFP) to measure cellular/mitochondria reduce/oxidize reaction
  • Calcein-based cell viability assay
  • Oxygen consumption in cultured cells
  • And many more!

 

Applications

Calcium Measurement

  • Intracellular free Ca2+
  • Mitochondrial Ca2+
  • Endoplasmic reticulum (ER) Ca2+ pool
  • Lysosomal Ca2+
  • Extracellular near membrane Ca2+

Simultaneous Measurement of intracellular a2+ and:

  • Cell volume
  • Cell contraction: Edge detection device
  • Membrane current: Patch Clamps
  • Phygocytosis
  • Cl-
  • Oxidase activation
  • Ca2+-CaM


Membrane Potential

  • Plasma membrane Potential
  • Mitochondrial Membrane Potential

pH

  • Intracellular pH
  • Phagosomal pH
  • Vacuolar pH
  • Lysosomal pH
  • More..

 

 

 

  • Lifetimes of very weak or diluted fluorophores
  • Complex, heterogeneous kinetics
  • Llifetime and time-resolved spectra of tyrosine and tryptophan fluorescence in proteins (frequency-doubler option required)
  • Micellar studies
  • Polymers
  • Membranes
  • Porphyrins and chlorophylls
  • Excited state electron and proton transfer
  • Analytical and environmental assays
  • Phosphors, LEDs and solid state devices
  • Fluorescence resonance energy transfer (FRET)
  • Time-resolved fluorescence anisotropy
  • More...

 

 

FluoDia PlateReader (click for more)

QuantaMaster UV VIS Rapid Excitation
(click for more)

 

Quantitative RNA measurement

  •  mRNA
  • RNA Invader assay
  • tRNA
  • rRNA
  • siRNA
  • Total RNA
  • PicoGreen RNA
  • RiboGreen RNA

Quantitative DNA measurement

  • dsDNA
  • ssDNA
  • PCR product

Blood sample based assays

  • Cholesterol (also for food samples)
  • Glucose
  • Glucose oxidase
  • Glutamic acid, glutamate
  • Monoamine oxidase
  • Cellulase
  • Acetylcholine and acetylcholinesterase
  • Phosphatidylcholine-specific phospholipase C, D sphingomyelinase
  • Respiratory burst in patient leukocytes
  • Lipid peroxidation

 

   Proteins

  • Quantitative protein measurement
    • Quantitative protein measurement
      • Protein Quantification
  • Detect enzyme activity of proteins
    • Detect enzyme activity of proteins
    • Tyrosine Kinas
    • Matrix metalloproteinases (MMP)
    • Elastase
    • L-asparaginase
    • Phosphatase 2A
    • b-galactosidase
  • GFP-based assay

   Cell based assays

  • Live/Dead/Viability/cytotoxicity
  • Factors affecting cell viability
  • Proliferation
  • Examining factors affecting cell proliferation in a large number of samples
  • Multi-drug resistance assay
  • Resistance of tumor cells to anticancer drugs
  • Phagocytosis
  • Factors affecting phagocytosis, phagocytotic deficiencies diagnosis
  • Adhesion assay
  • Factors affecting cell adhesion, including leukocyte, neutrophil, monocyte
  • Respiratory Burst
  • More...

 

  • Intracellular ion concentrations (Ca++, pH, etc.)
  • FRET
  • Membrane fluidity
  • Beta blockers
  • RNA and DNA
  • Membrane potential
  • Multiple probe experiments
  • Oxidants
  • Steady state fluorometry
  • More...