QuantaMaster™ UV VIS (click for more) |
QuantaMaster™ Luminescence (click for more) |
QuantaMaster™ NIR (click for more) |
- Millisecond kinetics
- DNA binding
- Intracellular ion concentrations
(Ca++, pH, etc.)
- Phosphorescence
- Membrane potential
- FRET
- Polarization/Anisotropy
- Stopped Flow
- Chemi-and bioluminescence
- Fluorescence kinetics
- Fluorescence ratios
- Excitation ratios
- Dynamic anisotropy
- Steady state
- Fluorescence
- Solid fluorescence
- Temperature dependant fluorescence
- Intracellular Ion Determination
(Ca++, pH, etc…)
- LRET
- Intrinsic Fluorescence of Proteins
- Photoluminescence of OLED devices
- Thin Films
- Single Photon Counting
- Analog Detection
- Quantum Yield studies of solids and solutions
- 4 Position peltier for higher throughput when doing long protein folding studies.
- Impurity detection
- Cell suspension work
- More...
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- Protein folding
- Phosphoresnce Lifetimes
- Fluorescence suppression
- Lanthanide emission and lifetimes
- Solid-state phosphorescence
- Delayed fluorescence
- Time resolved spectra
- Gated emission spectra
- Gated excitation spectra
- Microseconds to seconds kinetics
- DNA binding
- Intracellular ion concentrations
(Ca++, pH, etc...)
- Spectra and lifetimes of phosphors and inorganic materials
- Membrane potential
- FRET
- LRET
- Stopped Flow
- Chemi-and bioluminescence
- More...
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- Photochemistry
Singlet oxtgen is frequent byproduct
- Geology
NIR luminescence of minerals
- Forensic science
Identifying forged documents
- Photobiology and photomedicine
Singlet oxygen detection (1270 nm)
- Cancer treatment
Photodynamic therapy (PDT)
- Photobiology
Photodegradation caused by singlet oxygen
- Photosensitized oxidations
Photooxidation of environmental pollutants
- Optical fiber communication
Optical amplifiers
(e.g. chelated Er++ , 1540 nm)
- Agriculture
Development of environmentally friendly pesticides
- More...
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EasyRatioPro (click for more) |
RatioMaster™ (click for more) |
LaserStrobe™ (click for more) |
Calcium Measurement
- Intracellular free Ca2+
- Mitochondrial Ca2+
- Endoplasmic reticulum (ER) Ca2+ pool
- Lysosomal Ca2+
- Extracellular near membrane Ca2+
Membrane Potential
- Plasma membrane Potential
- Mitochondrial Membrane Potential
GFP & Variants
- Identifying cells coexpressing GFP for analysis
- Use mitoGFP to visualize mitochondria and measure mitochondrial calcium [Ca2+]m
- Use ratiometric GFP (redox-GFP) to measure cellular/mitochondria reduce/oxidize reaction
- HEK-293 cells transfected with the CaSR-pEGFP vector
- ECFP
- EYFP
Other Measurements
- Calcium imaging of
Single HEK 293 cell
- Taste Bud
- Pea Pollen tubes
- Respiratory burst & reactive oxygen species (ROS)
- NAD(P)H
- Changes in CaM availability
- Demonstration of selective plasma membrane permeabilization by digitonin in HEK293 cells
- Detection by immunofluorescence of PfCRT expression in HEK293 cells
- Receptor-mediated Glutamate release
(by following NADH signal)
- Fura-dextra
- Cellular GSH level
- FL-aldosterone efflux
- Latency of response to exogenous ATP or ADP
- Membrane traffic
- Liposome
- ER membrane
- Cell contractile activity & cell Size
- Cell Volume Measurement
- Phagocytosis
- Cell injury or death by PI fluorescent
- Metal induced cytotoxity
Other Ions
- Intracellular Mg2+
- Intracellular Mn2+
- Intracellular Zn2+
- Intracellular Sr2+
- Intracellular Na+
- Intracellular Cl-
- Cytosolic labile iron pool (LIP)
- Iron homeostasis
pH
- Intracellular pH
- Vacuolar pH
- Lysosomal pH
FRET
- Donor Excitation Spectroscopy
- Emission intensity
- Donor emission intensity
- Acceptor emission intensity
- Ratio of donor/acceptor emission intensity
- Measure molecular proximity
- Binding of enzyme to itssubstrate
- Measure Endoplasmic reticulum (ER) Ca2+
Calcium-related Measurement
- Ca2+ Waves
- Ca2+ wave quantitation
- Ca2+ wave induced bymechanical stimulation
- Ca2+ wave induced by mechanical wounding
- Ca2+ wave propagation
- In non-contacting cells
- Over a physical gap
- Ca2+ Oscillation induced by:
- Hg2+
- K+
- Heat
- ATP
- Dopamine
- FSH
- Caffine
- Ca2+ mobilization
- Ca2+ homeostasis
- Propagated cytoplasmic Ca2+cyt responses along the DVR wall.
- Simultaneous Measurement of intracellular Ca2+ and:
- Cell volume
- Phygocytosis
- Cl-
- Oxidase activation
- Ca2+-CaM
- More...
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Excitation-Shifted
Application |
Dye |
Calcium |
Fura-2,
Fura PE3
FFP18 Quin-2
Fura-Red |
| Sodium |
SBFI
BCECF
HPTS
SNAFL®
DM-NERF®
CI-NERF® |
| Potassium |
PBFI |
| Magnesium |
Mag-Fura-2
Mag-Fura-5 |
| Membrane Potential |
Di-4-ANEPPS
Di-8-ANEPPS |
Non-Ratiometric
Application |
Dye |
Calcium
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Fluo-3
Calcium Green™
Calcium Orange™
Calcium Crimson™
Rhod-2
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Chloride |
SPQ SPA
MQAE |
HIV protease
|
HIV protein substrate |
F-actin |
phallotoxins |
PKC |
Fim-1
Rim-1 |
Emission-Shifted
Application |
Dye |
Calcium |
Indo-1 |
| pH |
SNARF-1®
SNARF-2® |
| Magnesium |
Mag-Indo-1 |
| Membrane Fluidity |
Pyrene Fas
PATMAN |
| Free Fatty Acids |
ADIFABTM
|
| Membrane Potential |
JC-1 |
| Lipids, phoholipids |
Nile Red
|
| Nucleic acids |
Acridine
Orange |
Dual Dye
Application |
Dye |
Nucleic acids |
DAPI and FITC
FITC and APC
Fura Red™ and BCECF
FITC and Texas Red®
DAPI and PE |
Common FRET Pairs
EGFP/DSRed2 FRET
CFP/DsRed2 FRET
BFP/DsRed2 FRET
CoralHue M-Cyan/K-Orange
GFP/Rhod2
FITC/CY3 FRET
FITC/TRITC FRET
YFP/TRITC FRET
CY3/CY5 FRET
CFP/YEP FRET
BFP/GFP FRET
And Many More!
Applications
Calcium-related Measurement
- Ca2+ transients
- Ca2+ mobilization
- Ca2+ homeostasis
- Ca2+ waves
- Ca2+ oscillation
- Ca2+ spikes
- Ca2+ entry
- Capacitative Calcium Entry (CCE)
Other Ions
- Intracellular Mg2+
- Intracellular Mn2+
- Intracellular Zn2+
- Intracellular Sr2+
- Intracellular Na+
- Intracellular Cl-
- Intracellular Ba2+
- Divalent cation influx
- Labile iron pool (LIP)
- Iron homeostasis
- Na+/H+ exchange
- H+/HCO3- exchange
- Pf and PCO2
FRET
- Measure molecular proximity
- Binding of enzyme to its substrate
- Measure Endoplasmic reticulum (ER) Ca2+
- Donor Excitation Spectroscopy
- Emission intensity
- Donor emission intensity
- Acceptor emission intensity
- Ratio of donor/acceptor emission intensity
Other Measurements
- Respiratory burst & reactive oxygen species (ROS)
- NAD(P)H
- Nitric Oxide (NO)
- Intracellular GSH
- Identifying cells coexpressing GFP for analysis
- Use ratiometric GFP (redox-GFP) to measure cellular/mitochondria reduce/oxidize reaction
- Calcein-based cell viability assay
- Oxygen consumption in cultured cells
- And many more!
Applications
Calcium Measurement
- Intracellular free Ca2+
- Mitochondrial Ca2+
- Endoplasmic reticulum (ER) Ca2+ pool
- Lysosomal Ca2+
- Extracellular near membrane Ca2+
Simultaneous Measurement of intracellular a2+ and:
- Cell volume
- Cell contraction: Edge detection device
- Membrane current: Patch Clamps
- Phygocytosis
- Cl-
- Oxidase activation
- Ca2+-CaM
Membrane Potential
- Plasma membrane Potential
- Mitochondrial Membrane Potential
pH
- Intracellular pH
- Phagosomal pH
- Vacuolar pH
- Lysosomal pH
- More..
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- Lifetimes of very weak or diluted fluorophores
- Complex, heterogeneous kinetics
- Llifetime and time-resolved spectra of tyrosine and tryptophan fluorescence in proteins (frequency-doubler option required)
- Micellar studies
- Polymers
- Membranes
- Porphyrins and chlorophylls
- Excited state electron and proton transfer
- Analytical and environmental assays
- Phosphors, LEDs and solid state devices
- Fluorescence resonance energy transfer (FRET)
- Time-resolved fluorescence anisotropy
- More...
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FluoDia PlateReader (click for more) |
QuantaMaster UV VIS Rapid Excitation
(click for more) |
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Quantitative RNA measurement
- mRNA
- RNA Invader assay
- tRNA
- rRNA
- siRNA
- Total RNA
- PicoGreen RNA
- RiboGreen RNA
Quantitative DNA measurement
Blood sample based assays
- Cholesterol (also for food samples)
- Glucose
- Glucose oxidase
- Glutamic acid, glutamate
- Monoamine oxidase
- Cellulase
- Acetylcholine and acetylcholinesterase
- Phosphatidylcholine-specific phospholipase C, D sphingomyelinase
- Respiratory burst in patient leukocytes
- Lipid peroxidation
Proteins
- Quantitative protein measurement
- Quantitative protein measurement
- Detect enzyme activity of proteins
- Detect enzyme activity of proteins
- Tyrosine Kinas
- Matrix metalloproteinases (MMP)
- Elastase
- L-asparaginase
- Phosphatase 2A
- b-galactosidase
- GFP-based assay
Cell based assays
- Live/Dead/Viability/cytotoxicity
- Factors affecting cell viability
- Proliferation
- Examining factors affecting cell proliferation in a large number of samples
- Multi-drug resistance assay
- Resistance of tumor cells to anticancer drugs
- Phagocytosis
- Factors affecting phagocytosis, phagocytotic deficiencies diagnosis
- Adhesion assay
- Factors affecting cell adhesion, including leukocyte, neutrophil, monocyte
- Respiratory Burst
- More...
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- Intracellular ion concentrations (Ca++, pH, etc.)
- FRET
- Membrane fluidity
- Beta blockers
- RNA and DNA
- Membrane potential
- Multiple probe experiments
- Oxidants
- Steady state fluorometry
- More...
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