[Ca++]i = Kd * (R-Rmin)/(Rmax-R) * (Sf2/Sb2)
where R is the ratio of the fluorescence intensities obtained with excitation at 340 nm and 380 nm; Rmin and Rmax are the limiting ratios, and Sf2 and Sb2 are the limiting 380 nm fluorescence intensities in the absence of Ca++ and in the presence of saturating amounts of Ca++ respectively.
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Streamlined Capture Interface
Places frequently used capture controls within easy reach. AutoSet feature assists in setting color balance exposure parameters. Back to top.
High Speed Acquisition
IM supports High Speed streaming of images into system memory. During streaming the DeltaRam or DG4/DG5 will lock to the camera frame transfer or exposure camera outputs. Back to top.
Streaming Memory
The number of frames that can be acquired is dependent on the amount of system memory. The amount of allocated memory can be assigned in the Digital Camera control interface under the configure button. The streaming tab will show the current amount of memory that has been reserved from the driver to use for preview and fast acquisition. Back to top.
Shutter Controls
The shutter open and close buttons have been added to the toolbar to improve convenience. When in mono access dialog the space bar will also open and close shutter. Back to top. |
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Close Shutter |
Open Shutter |
Experimental Clock
An experimental clock with options and remote control abilities is now included. Acquisitions can be controlled through the clock.  Back to top. |
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FRET Acquisition Module
The crosstalk constants can be input manual or an automation will calculate these constants and input values into dialog box. Donor and Acceptor Fluorophores can be selectable from popup menu. User is prompted to pick appropriate AOI file for measurement of FRET Users have the choice of 3 Normalization methods.
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Image Overlay
Overlay assists in tiling, stitching and aligning images. Back to top. |
Click on image to enlarge. |
Workflow Management
Workflow management creates customized workflows and toolbars Back to top. |
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Stitching
New stitching and tiling features allow you to build a large image from multiple, smaller acquisitions
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Alignment Correction
Alignment correction allow you to align individual or sequence images for translation, rotation, or scaling
Useful for images taken over time, images with camera movement, or to align channels in multiple images from a beam splitter. Back to top. |
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Dye Management
Allows application of LUTs to monochrome fluorescence images.
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Local Zoom
Mouse over zoom of active image. Up to 1600% magnification
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Imaging Enhancement & Processing
Maximize brightness/contrast, reduce artifacts, separate objects from background, and enhance non-visual data (data visible to camera but not eye)
Click on image to enlarge.
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Imaging Overlay
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Problem
The observed details are limited. Back to top. |
Solution
Overlay fluorescent and transmitted light images (phase contrast, DIC, polarized light) in one color image. |
FFT Algorithm
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Problem
This image shows a periodic source of noise Back to top. |
Solution
Applying FFT algorithm removes the periodic noise. |
Image Thresholding
Select appropriate morphometric characteristics to be used for thresholding.
Identify regions of interest to be analyzed. Mask regions that fell out of the threosholding range.
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Calibration Wizards
Calibration Wizards assist you in creating reference spatial calibrations and calibrating a single image from a spatial reference in an image. Back to top.
Spatial and Intensity Measurement
Analyze and calibrate radiolabeled, stained, luminescent and fluorescent gels and blots. Improve quantification. Back to top.
Intensity Tracking
Unlimited number of regions of interest can be drawn and the average intensity can be measured within each region.
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Auto Splitting
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Reporting Data
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Software
