A wide variety of accessories are available for your systems, and the list is constantly growing. These accessories include monochromators, polarizers, computer-controlled four-sample holders, solid sample holders, dewars, red-sensitive PMTs, and much more.
Extended Red PMT to 900 nm
Extended red PMT option available at the time of original order. Extends the emission wavelength range to approx. 900 nm. Should be ordered for samples emitting above 600 nm. In the UV-VIS closely follows the sensitivity curve for the regular PMT detector. Especially recommended for:
- chlorophyll
- porphyrins and phtalocyanines
- rhodamine-based dyes
- NIR DNA probes
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Stopped Flow Accessory
The stopped flow accessory is used to rapidly mix small volumes of two (or more) different chemicals in a cuvette, quickly stop the flow of chemicals to the cuvette and monitor the resulting chemical reaction via optical means. In some instances, the chemical reaction will result in luminescence and this optical signal can be monitored using a fluorometer. In other instances, the chemical reaction only produces a change in the optical absorption properties and must be monitored using an absorption technique. The primary experimental interest is in the speed of the chemical reaction following the mixing in the cuvette, in addition to the spectral properties of the resulting absorption and/or luminescence.
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Four-position Sample Holder
The muli-position Peltier, featuring a four-position turret and magnetic stirring can accommodate standard cuvettes or microcuvettes. The automated sample holder has a controllable temperature range of -20°C to 105°C.
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Powdered Sample Holder
The powdered sample holder head easily adapts to the solid sample base with one thumbscrew adjustment. The sample head can be disassembled for easy sample loading and cleaning.
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Front Face Solid Sample Holder
The front face solid sample holder, capable of both linear and rotational travel, was designed for the measurements of solid compounds, microscope slides, or films. The solid sample holder head mounts onto a base that can be removed easily to substitute a powder sample holder head.
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Titrators
Titrations are performed to measure a number of biochemical and physical parameters, including binding constants, stoichiometry and kinetics. PTI offers fully automated titration solutions that are integrated into the software. Parameters such as mixing, volume, speed, and calibration are dictated in the software and can be adapted to your needs.
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Intergrating Sphere
Redesigned for enhanced measurement of quantum yields of solids, films, and powders. We use a 6-inch diameter sphere and attach it directly to the sample chamber on the port opposite the excitation channel. This design minimizes the effect of the excitation, emission, and sampling ports on the accuracy of the measurement. We also changed the optics inside the sample compartment to refocus the excitation beam inside the sphere by adding two optics inside the sample. One of these optics is adjustable in both X and Y planes to translate the focus inside the sphere. To collect the emission channel, a fiber brings the light from a port on the sphere into the emission monochromator through the second entrance port. A reflectively coated sample holder positions the sample in focus at the center of the sphere to provide superior direct illumination.
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Single Cuvette Peltier
The Peltier provides unmatched temperature stability and accuracy over the controllable temperature range of -20 ºC to 105 ºC. Software selectable temperature ramping is established by setting the starting and ending temperatures in addition to the rate of change. Data points are measured in steps defined by the temperature increment. The minimum increment value is 0.1 ºC. Temperature is measured by a probe inserted into the sample cuvette and the actual sample temperature is constantly displayed on screen in real-time. The maximum temperature ramping speed is 20 ºC/minute with magnetic stirring.
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Muscle Strip
The muscle strip is inserted into a standard 1 cm cuvette, combining the lower muscle hook with unique perfusion tubes, a tension transducer with upper muscle hook, and an interface electronic control unit. The accessory can be used with any cuvette-based fluorescence system having a standard single cuvette holder complete with tension transducer and transducer mounting bracket with micrometer position adjustment.
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Polarizers
PTI offers a wide variety of polarizers ranging from manual sheet polarizers (click here to view graph) to automated large aperture Glan Thompson polarizers (click here to view graph). All configurations allow for automated software control, automatic G factor determination, and real-time acquisition of HH, VH, VV, and HV analysis. Measure steady state anisotropy in single emission configuration or dynamic anisotropy utilizing our dual emission configuration.
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Cold Finger Dewar
The cold finger dewar is designed to be used with liquid nitrogen as coolant (77K). It can also be used with organic solvent slushes at discrete temperatures above 77K. Includes: quartz cold finger dewar that accepts 5 mm tubes, dewar holder for the sample turret or single cuvette holder, foam lid for the dewar and extension collar with altered sample chamber lid, and a sample compartment. The dewar features a Suprasil quartz cold finger that passes light down to about 200 nm. Samples are placed in NMR and EPR tubes and the liquid nitrogen placed in the dewar will typically last several.
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Remote Sensing Accessory
The remote sensing accessory allows in vitro or in vivo measurement by means of a quartz bifurcated fiber bundle or Liquid Light Guide. One fiber leg is attached to the second exit port of the excitation monochromator to provide excitation light to the sample. The second leg is attached to an open entrance port of the emission monochromator to detect the fluorescence signal emitted from the sample.
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Selections of Long-pass Filters
Long-pass filter sets provide spectral discrimination for the emission channel and prevent the excitation light from being detected by the PMT. Filters should be selected so they cut off unwanted wavelengths, such the second order. The filters should be used with a filter holder (2 filter holders included with instrument).
- The longpass filter set includes 1" round filters with cut-off wavelengths of 345, 400, 445, and 515 nm
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Bandpass Filters
10-nm bandpass interference filters are available individually or as a set. The peak transmittance wavelengths are 280, 289, 297, 308, 334 nm and from 340 nm to 650 nm at 10-nm increments. The filters should be used with a filter holder (2 filter holders included with instrument). The bandpass filters are especially recommended for:
- FRET experiments when looking at donor fluorescence
- samples which exhibit fluorescent tautomeric forms
- excimer and exciplex systems, when looking at the monomer emission band
- molecules, which undergo excited state proton transfer (ESPT) or intramolecular charge transfer (ICT, TICT) and exhibit dual fluorescence bands
- experiments, which require elimination of unwanted emission at longer wavelengths
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Neutral Density Filters
Neutral density filter set. The neutral density filter set consists of six 1" round filters with approximate optical densities of 0.1, 0.3, 0.5, 1.0, 2.0 and 3.0 suitable for a spectral range of 300-2000 nm. The filters should be used with a filter holder (2 filter holders included with instrument). The neutral density filters are used to decrease the signal intensity without changing the width of the emission slit.
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Semimicro Cuvette The semi-microcuvette cuvette and insert lets the end user save money and time by using smaller volumes of samples while maintaining the correct optical height.
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Circulating Water Bath
coming soon
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