Sensitivity: Capable of measuring lifetimes of 7pM fluorescein
Range of Measurable Lifetimes: 100 picoseconds to ~20 microseconds (sample intensity dependent)
Laser Illuminator
- Wavelength Range: 360 to 990 nm (depending on dye used) (235 to 345 nm with optional frequency doubler)
- Bandwidth:0.04 nm
- Pulse Width: 800 picoseconds
- Peak Power: 275 KW at 5 Hz
- Pulse Energy: 220 microjoules per pulse at 500 nm, 5 Hz
- Repetition Range: 2 to 20 Hz
Emission Monochromator
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Wavelength Range: 180 nm to 24 microns, continuously tunable (useful detection wavelength range dependent on grating and photomultiplier tube)
- Bandwidth: 0 to 25 nm, continuously tunable
- Resolution: 0.2 nm
- Accuracy: +/- 0.5 nm
- Reciprocal Linear Dispersion: 4nm/mm
- Throughput: 60% at 400 nm
- Stray Light: <0.02% two bandwidths from 365 nm Hg line
Stroboscopic Detector
- Wavelength Range: 185 to 680 nm with standard R1527 PMT
Excitation Source
Variable frequency pulsed nitrogen laser/tunable dye laser combination
Wavelength range: 337 nm to 990 nm using various dyes
Three standard dyes included, additional dyes available
Provisions for tuning the dye laser under computer control
Optional frequency doubler module is available from about 345 nm down to 235 nm.
Spectral bandwidth: 0.4 Angstroms (0.04 nm)
Can excite fine spectral structure, also further reduces stray light
Pulse width: app. 800 ps
For easy deconvolution lifetimes to ca. 100 ps
Pulse energy (PLD500 at 500nm): 220 μJ typ.
Peak power at 5 Hz (PLD500 at 500nm): 275 kW
Power allows for the measurement of very weak samples
Repetition rate: variable, up to 20 pulses per second
Allows measuring lifetimes and time-resolved spectra of photosensitive emitters with no photodecomposition
Easy to use; reliable with minimum routine maintenance
The excitation source is coupled to the sample compartment via a 350-µm-diameter quartz fiber optic cable
Storage cassette and fiber micro-positioner included
Sample Compartment:
- Four ports to accommodate various light sources and detectors
- Configurable in L-, T- or X-formats
- Quartz optics for optimum sample illumination and emission signal collection
- Roomy compartment permits easy access and accommodates various options
- Thermostatable 10 x 10 mm cuvette holder (requires external water bath, not included)
- One 10 x 10 mm quartz cuvette included
- Variable speed sample stirrer
- Lid-activated emission shutter to protect sensitive detectors
- Excitation and emission filter holders accommodate 2 in. square or 1 in. diameter round filters
- Provision for injection into sample
- Gas purgeable compartment
Detector
- Stroboscopic PMT detector housing with 1527 photomultiplier tube
- Built in avalanche and stripline detector circuits
- Wavelength range 185 to 650 nm
- Extended wavelength range option to 900 nm available
- Special systems for detection beyond 900 nm (inquire about PTI custom designed IR systems)
Computer, Software and Electronics
FeliX32™ Advanced Fluorescence Analysis Software Package
including 1-4 exponential, global analysis, stretched exponential, micelle kinetics, anisotropy decays, MEM and ESM lifetime distribution analysis, all above with deconvolution capability.
All appropriate electronics included
BryteBox™ computer interface included
and workstation included.
Data Acquisition Characteristics
Sensitivity allows for the measurement of lifetimes of 7 pM fluorescein
Minimum measurable lifetime: <100 ps
Can measure fluorescence using either linear or non-linear (i.e. arithmetic progression or logarithmic) time base; can measure complex decays with lifetimes from <100 ps to hundreds of ns within a single acquisition
Direct acquisition of Time-Resolved emission spectra (TRES)
Fast, accurate data collection rates, <30 seconds typ.
OTHER
Euro version CE approved |
System
Specifications
