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Applications
FluoDia T70 can be used to measure biological samples from all species, from bacterial, yeast, worms, flies, animals to human. It can be used to measure all types of biological samples, from DNA, RNA, protein, whole cell to blood samples. It can be used to measure non-biological samples too.
FluoDia T70 can be used for microplate based assays such as the following:
Quantitative RNA measurement
- mRNA
- RNA Invader assay
- tRNA
- rRNA
- siRNA
- Total RNA
- PicoGreen RNA
- RiboGreen RNA
Quantitative DNA measurement
Blood sample based assays
- Cholesterol (also for food samples)
- Glucose
- Glucose oxidase
- Glutamic acid, glutamate
- Monoamine oxidase
- Cellulase
- Acetylcholine and acetylcholinesterase
- Phosphatidylcholine-specific phospholipase C, D sphingomyelinase
- Respiratory burst in patient leukocytes
- Lipid peroxidation
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Proteins
- Quantitative protein measurement
- Quantitative protein measurement
- Detect enzyme activity of proteins
- Detect enzyme activity of proteins
- Tyrosine Kinas
- Matrix metalloproteinases (MMP)
- Elastase
- L-asparaginase
- Phosphatase 2A
- b-galactosidase
- GFP-based assay
Cell based assays
- Live/Dead/Viability/cytotoxicity
- Factors affecting cell viability
- Proliferation
- Examining factors affecting cell proliferation in a large number of samples
- Multi-drug resistance assay
- Resistance of tumor cells to anticancer drugs
- Phagocytosis
- Factors affecting phagocytosis, phagocytotic deficiencies diagnosis
- Adhesion assay
- Factors affecting cell adhesion, including leukocyte, neutrophil, monocyte
- Respiratory Burst
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Other specific assays
- RNA Invader assay (Invader® is a registered trademark of Third Wave Technologies Inc.)
- Fluorescence-based thermal shift assay
Click here to view Thermal Shift Protocol (696 KB PDF)
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- ThermoFluor Binding assay. (ThermoFluor® is a registered trademark of 3-Dimensional Pharmaceuticals, Inc.)
- Monitor fibrillogenesis in vitro
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- Protein Melting Temperature
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- Fluorescence Resonance Energy Transfer (FRET)
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Click here to read more on FRET applications.
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