Linearity

Correlation coefficiency ≥ 0.995

Sensitivity

2.4 fmol/well fluorescein; 4-MUF: 0.1 pmol/well

Reproducibility

CV £ 1.5%

Dark level

≤ 3 x 10^-4% full scale

Stability

≤ 3 x 10^-4% full scale/8 hours

Dynamic range

0 –16,777,215 counts

Gating time

10 msec–10 sec (can be setup for every 10 msec)

Measurement time

Approx. 51 sec/96 wells (if measured per well)

Dual wavelength

Approx. 67 sec/96 wells (if measured per plate)

Single wavelength

Approx. 40 sec/96 wells , Approx. 110 sec/384 wells

Number of averages

1–100 times/well

Fluorescence intensity calibration

Automatic calibration using the exclusive calibration plate

Sample mixing

Linear and butterfly mixing.  Time and velocity software controlled.

Temperature control

     Range

Room temperature +5°C–75°C

     Heating stage

Flat heater plate, 96-well or 384-well heater plate, dual P.I.D. control. 

     Thermal fuse

Built-in (123°C)

     Incubation method

Temperature stepping, simple incubation or incubation

     Accuracy

± 0.3°C

     Homogeneity

± 0.3°C

     Rise time

≤ 6 min. (up to 75°C from R.T.)

Temperature rise time of sample

(rise time from 20°C to 68°C)
≤ 5 min. without pre-heating
≤ 3 min. if pre-heated at 45°C
≥ 1 min. if pre-heated at 68°C

Plate formats

6-384 well plates, strip tubes, 1536 well plates (optional)

Plate Dimensions

     Width

86.0 ± 0.8 mm

     Length

128.0 ± 0.8 mm