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RatioMaster™ Accessories
Single Channel Photometer
High sensitivity PMT detector:
- Photon counting mode: for low signal, as in most ratio fluorescence measurement.
- Analog modes: for high signal
- Detector wavelengths: UV- NIR with appropriate PMT
Precise field-of-view control
- Precise field-of-view control
- A viewing eyepiece for Parfocal viewing.
An adjustable aperture for selecting the area of interest.
- Easy to switch from View to Measure via a flipping mirror.
Connect to the microscope on any of the available ports via a C-mount adaptor.
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 Dual Channel Photometer
Has all the properties of a single channel photometer, plus:
Allows for simultaneous detection of two emission wavelengths by means of two independent PMT detectors. Has provision for beam splitter and dichroic cube for emission wavelength selection. Dichroic assembly is placed within the photometer for selection of the two emission wavelength ranges. Provides high-speed (millisecond) detection for emission-shifted probes. Data acquisition rates of up to 1000 ratios per second are possible. Back to top.
 Dual C-Port Adaptor with Flipping Mirror
The IM-9 is a Dual C port adaptor for microscope with flipping mirror allowing user to select between two different emission detection devices, for example camera and photometer. Primarily used to mount two optical detectors one output for photometer, another output for camera. Both ports are identical. The flipping mirror allows easy and rapid switch between the two detectors without a hardware change. Good for sequential, not simultaneous, measurement. Very useful to retrofit old fluorescence scopes. Attaches directly to the C-mount on a microscope. Provides one input and two standard C-Port outputs. Back to top.
Fluorescence Lifetime Upgrades
Pulsed laser and flash lamp excitation sources and detectors can be added to your microscope to measure Fluorescence and phosphorescence lifetimes. FeliX32™ advanced analysis software is used for curve fitting the decay data. Dual View Image splitter module The IM-5B adds popular dual Image splitter modules to your EasyRatioPro system. This allows for emission ratio and FRET Imaging applications like Indo-1, JC-1 membrane potential or CFP/YFP FRET.
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Dual View Image Splitter Module
The IM-5B adds popular dual Image splitter modules to your EasyRatioPro system. This allows for emission ratio and FRET Imaging applications like Indo-1, JC-1 membrane potential or CFP/YFP FRET.
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Dual C Port Adapter for Edge detection, Fluorescence and Electrophysiology
PTI's new Dual C-Port adapters attach directly to the C-Port of any microscope. They provide one input and two standard C-Port outputs. Both ports have access to full microscope field of view while a red dichroic mirror separates light to two output ports for above and below 620 nm. PTI's model D104 microscope photometer is attached to the port with under 620 nm and CCD camera to the port above 620nm. The aperture on the model D104 is down field from the red dichroic so fluorescence may be collected from a smaller area than the image. The Dual C-Port adapter allows simultaneous viewing of fluorescence and bright field with trans-illuminator on and 660nm long pass filter installed. (NOTE: emission filter for D104 MUST be placed in D104B NOT in microscope) Common applications include edge detection, fluorescence, electrophysiology, and any other application where view of full field during fluorescence measurements is advantageous.
Red Short Pass Dichroic Mirror,
Scan Range
450nm to 850nm
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 |
| Number of Inputs |
1 |
| Number of Outputs |
2 (1 front and 1 side) |
| F-number |
4 |
| Magnification Factor |
1 |
| Mount Type |
C-port (input/output) |
| Dichroic Mirror |
Red Short-Pass Beamsplitter,
Cutoff
Wavelength = 620 nm |
| Flipping Mirror |
Aluminum coated, MgF2 overcoat,
50mm x 50mm x 2mm |
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Imaging Subsystem Add-Ons
The EasyRatioPro add-on to your imaging system. It is ideal to measure very fast or ultra low light levels. EasyRatioPro subsystem includes high sensitivity CCD or EMCCD camera, electronics and EasyRatioPro software package and Labmaster interface. May require IM-9 dual C port adaptor, in order to accommodate both photometer and camera.
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Accessories and add-ons
- Dual channel High speed Photometry with FeliX32™
- Dual C-Port adapters
- Fluorescence and Phosphorescence lifetime upgrades
- Dual Imaging and dual splitter modules for emission shifted dyes and FRET applications
- Imagemaster 5 analytical image software
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Accessories
Specifications
