When you need to perform microscope photometry, what do you use? Most labs end up acquiring some microscope accessory intended for another purpose--such as photography.
Unfortunately, these devices are rarely up for the task. Microscope photometry requires absolute light-tightness. It also helps to have a device designed to accommodate a photomultiplier housing--or even two. Additionally, it should hold filters, have an adjustable iris, and provide a means to view the image being measured.
Microscope photometers from PTI do all of this--and more. They are specifically designed for sensitive photometric measurements. And they couple easily to virtually any popular microscope.
The Model D-104B is a single-channel photometer. The D-104C is a dual-channel photometer that allows simultaneous measurements at two different wavelengths via a dichroic cube.
No system is complete without an analog/digital photon counting detector
 In order to complete a Ratiomaster
system, an analog/digital photon counting
detector, selected from only our best
quality and performing photo-multipliers,
the 814 series. You may also select one of
the excellent Red sensitive PMT's to complement
the system.
Analog/Digital Photon Counting
Model 814 is a compact PMT housing
for analog or digital (photon-counting)
detection. The detection mode is
switchable, and the built-in LCD can also
selectively display high voltage or analog
signal level.
Features power on/off switch, high
voltage adjust, LCD high voltage/analog
signal display, detection mode and display
mode switches, gain, time constant and
offset adjustment controls, BNC signal
output connection, BNC external high voltage
adjust input connection, light-tight RF shielded case, internal direct anode output
mode control, and socket for most 1-1/8
inch side-on photomultiplier tubes. Includes
a separate wall-mount line voltage
power supply. This detection system excels
at applications like the following:
- Live-cell imaging
- High-speed emission ratio imaging
- Quantitative FRET, FRAP, FISH
- Luminescence
ANALOG MODE:
This mode can
be used for medium to high levels of
light intensity. The maximum PMT current
is 10 microamperes. The output is a
DC signal between +13 Volts and -13
Volts.
PHOTON-COUNTING (DIGITAL)
MODE:
This mode can be used for low
to medium light levels up to 4 million
counts per second. The output is a TTL
level, active low signal. The signal line
must be terminated at 50 ohms. The
output is a frequency corresponding to
the number of photons impinging on
the photocathode.
Photometer Specifications
- DIGITAL SECTION
- Maximum Count Rate: 4MHz
- Pulse Pair Resolution:250ns
- Output: TTL level active low pulse
- Rise Time: 20ns
- Fall Time: 100ns
- Pulse Width: 220ns
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Hardware
Specifications
