Buyer's Guide
Each of PTI's diverse fluorometer systems is designed with particular user needs in mind. The QuantaMaster™ UV VIS spectrofluorometer is PTI's basic bench-top fluorometer, and is designed for the user who wants:
- an easy-to-use and reliable bench top fluorometer that has the best sensitivity for quick and accurate measurement of fluorescence or phosphorescence signals.
- a bench-top fluorometer with the unique additional versatility that only PTI open architecture provides.
- the best all-around spectrofluorometer for the majority of applications
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General Applications
- Millisecond kinetics
- DNA binding
- Intracellular ion concentrations (Ca++, pH, etc.)
- Phosphorescence
- Membrane potential
- FRET
- Polarization/Anisotropy
- Stopped Flow
- Chemi-and bioluminescence
- Fluorescence kinetics
- Fluorescence ratios
- Excitation ratios
- Dynamic anisotropy
- Steady state
- Fluorescence
- Solid fluorescence
- Temperature dependant fluorescence
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- Intracellular Ion Determination (Ca++, pH, etc.)
- LRET
- Intrinsic Fluorescence of Proteins
- Photoluminescence of OLED devices
- Thin Films
- Single Photon Counting
- Analog Detection
- Quantum Yield studies of solids and solutions
- 4 Position peltier for higher throughput when doing long protein folding studies.
- Impurity detection
- Cell suspension work
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General Information
The QuantaMaster™ is an easy-to-use, open architecture spectrofluorometer with extremely high sensitivity.
The QuantaMaster™ UV VIS fluorometer is very easy to use. Just insert the sample and the measurement and analysis are all under keyboard control. One can even automate measurement by means of easy to write macros.
With a signal to noise of 10,000:1, the QuantaMaster™ UV VIS is the most sensitive bench-top fluorometer on the market today. Sensitivity in a fluorometer determines the ultimate analyte detection limit. Sensitivity defines: how small a quantity can be detected, how long the measurement will take and how accurate the measurement will be. The ability to measure minute amounts of a substance can also save money, particularly in situations where expensive reagents or precious biological materials are used.
The QuantaMaster™ UV VIS is unique as a bench top fluorometer in that it offers a modern open architecture design, which is normally only found in much higher priced research grade fluorometers. The open architecture design offers some great benefits, such as: accommodating more options, capability of other fluorescence measurements such as very short lifetimes, coupling to a microscope, and is easily upgraded.
A continuous high-pressure xenon lamp provides a virtual point source broadband excitation illumination. Excitation and emission wavelengths are selected by means of auto-calibrated, computer-controlled, scanning QuadraScopic™ monochromator with continuously variable slits. QuadraScopic™ monochromators come with two entrance and exit ports that provide for additional excitation sources and/or detectors.
Also included as standard in the roomy QuadraCentric™ sample compartment are: a 10 x 10 mm thermostatable cuvette holder equipped with a variable speed stirrer, high efficiency quartz optics, filter holders, active excitation correction, lid activated emission shutter and one quartz cuvette. In addition, there is plenty of room for numerous options, such as: polarizers, solid or powdered sample holder and other accessories. The system offers two types of detection: analog for measuring highly fluorescent substances and photon counting for very weak fluorescence.
Installation, software, computer, interface and everything that you need in a turnkey system are included in the basic price. Optionally, you may supply your own computer for data acquisition and reduce the price of the system.
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Optical Layout and Physical Dimensions

Key to Figure
1 Arc lamp housing
2 Adjustable slits
3 Excitation monochromator
4 Sample compartment
5 Baffle
6 Filter holders
7 Excitation/emission optics
8 Cuvette holder
9 Emission port shutter
10 Excitation correction unit
11 Emission monochromator
12 PMT detector |
Dimensions
(excluding computer and electronics)
System overall:
32 x 26 inches
82 x 67 cm
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