Detection limit |
460 attomplar fluorescein in 0.1 M NaOH
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Signal to noise ratio |
10,000:1 or better
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Minimum measurable lifetime |
400 ns |
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Data acquisition rate |
1000 points/sec. to 1 point/100 sec |
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Wavelength range |
180 nm to 24 microns, continuously tunable (useful illumination wavelength range dependent on grating and lamp; useful detection wavelength range dependent on grating and photomultiplier tube)
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Bandwidth |
0 to 25 nm, continuously variable |
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Detection range |
185 to 680 nm with standard 1527 PMT (extended range to Near IR available) |
The sensitivity of the QuantaMaster™ UV VIS fluorescence spectrometer was assessed by testing fluorescein solution in 0.1 N NaOH. Equipped with sensitivity enhanced optics, the fluorescein spectrum is clearly detectable in the attomolar range under the experimental conditions.
Lamp type |
75 W xenon lamp standard (can accommodate 100 W Mercury, 100 W Tungsten-Halogen or 150 W Xenon lamps) |
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Lamp housing |
No cooling or ozone venting required |
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Reflector |
Thermally-matched front-surface ellipsoidal reflector with 67% efficiency |
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Power Supply |
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Line voltage regulation |
0.1% current variation for 5 volt line change |
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Load regulation |
0.1% current variation for 50% change in load impedance |
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Stability |
0.2% (after warm-up) |
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| Igniter (intergrated) |
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Start pulse |
30 kV |
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Starter |
Highly shielded to prevent RF interference to computer and
peripherals |
Excitation wavelength range |
330–650 nm |
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Optimum scanning range |
290–610 nm |
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Wavelength selection speed |
< 2 milliseconds point-to-point |
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| Resolution |
< 1 nm
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| Accuracy |
+ 3/-1 nm |
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| Wavelength bandwidth |
Adjustable from 0–24 nm |
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| Stray light rejection |
> 104 |
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| Light delivery |
0.5 meter quartz fiber optic bundle |
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| Liquid light guide |
2 meter length 2 mm core diameter |
Ports |
Four ports to accommodate various light sources and detectors |
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Configurations |
L, T or X-formats |
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Optics |
Quartz optics for optimum sample illumination and emission signal collection |
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Cuvette |
One 10 x 10 mm quartz cuvette included |
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Cuvette holder |
Thermostatable 10 x 10 mm cuvette holder (requires external water bath, not included) |
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Sample stirrer |
Variable speed |
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Emission shutter |
Lid-activated emission shutter to protect sensitive detectors |
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Filter holders |
Excitation and emission filter holders accommodate 2 inch square or 1 inch diameter round filters |
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| Sample injection |
Yes |
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| Gas purgeable |
Yes |
QuadraScopic™ monochromator |
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Focal length |
200 mm |
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Aaperture ratio |
f/4 |
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Optical configuration |
Czerny-Turner configuration |
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Wavelength range |
180 nm to 24 microns with appropriate grating |
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Bandpass |
Continuously adjustable from 0 to 25 nm |
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Resolution |
0.5 nm |
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Accuracy |
+/-1 nm (using manual wavelength control)
+/- 0.25 nm (using motorizing option under computer control) |
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Reproducibility |
+/-1 nm |
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Minimum step size |
0.25 nm |
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Reciprocal linear dispersion |
4 nm/mm |
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Throughput |
60% at 300 nm |
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Scattered light |
0.02% two bandwidths from 365 nm Hg line |
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Optical path height |
76 mm |
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Grating size |
50 x 50 mm |
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| Gratings |
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Emission grating |
1,200 l/mm blazed at 400 nm |
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| Options |
An extensive selection of gratings, ruled and holographic, optimized from 75–2400 grooves/mm are available |
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Monochromator Options |
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Slits |
Optional computer-controlled (patent pending) slits or micrometer slits |
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Dual gratings |
To efficiently cover an extended wavelength range |
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Double monochromators |
Double monochromators for lower stray light (useful for highly scattering samples such as suspensions or solids) |
Photomultiplier tube detector |
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Wavelength range |
185 to 680 nm |
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Extended wavelength range |
Up to 900 nm (optional) |
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Detection options |
Analog (high signal levels) or photon counting (low signal levels) detection options, directly selectable on the photomultiplier housing. |
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High voltage range |
200 to 1,100 VDC, manually adjustable |
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Temperature coefficient |
± 0.01% max. (+5 to 40°C) |
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Drift |
± 0.03%/hr. max. (after 15-minute warm-up) |
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Photon Counting Mode |
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Maximum count rate |
3.8 MHz |
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Pulse pair resolution |
265 ns |
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| Rise time |
20 ns |
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Fall time |
100 ns |
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Pulse width |
220 ns |
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Analog Mode |
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Inherent noise |
50 pA p-p typically (0.05 millisecond time constant) |
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Offset correction |
± 50 nA |
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Gain settings |
1, 0.1, 0.01, 0.001 x mA equals 1 volt |
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Time constant settings |
0.05, 0.5, 50 or 500 milliseconds |
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Options |
|
Detection range |
Optional
PMT available for detection between 700 to 900 nm |
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| Cooled PMT housing |
Optional cooled PMT housing available for IR detection noise reduction |
Light sources |
Optional special light sources such as lasers for specialized applications available |
