QuantaMaster
UV VIS
Rapid Excitation
QM 8 RAM

Basic Specifications

Signal Detection Limit:
The sensitivity of the QuantaMaster™ UV VIS fluorescence spectrometer was assessed by testing fluorescein solution in 0.1 N NaOH.  Equipped with sensitivity enhanced optics, the fluorescein spectrum is clearly detectable in the attomolar range under the experimental conditions.

Signal to Noise Ratio: 10,000:1 or better
Data Acquisition Rate: 1000 points/sec. to 1 point/100 sec.
Wavelength Range: 180 nm to 24 microns, continuously tunable (useful illumination wavelength range dependent on grating and lamp; useful detection wavelength range dependent on grating and photomultiplier tube)
Bandwidth: 0 to 25 nm, continuously variable
Detection Range: 185 to 680 nm with standard 1527 PMT (extended range to Near IR available)
High Voltage Range: -200 to -1,100 VDC
Resolution: 0.2 nm
Accuracy: +/- 0.5 nm

Excitation Source

• High efficiency continuous illumination system:
• 75W xenon lamp provides virtual point source of broadband illumination
• Can accommodate 100W Mercury, 100W Tungsten-Halogen or 150W Xenon lamps
• IR filter provided in the excitation path to prevent thermal damage to system
• Wavelength range of illumination (with the IR filter in place) 200 to 650 nm

Power supply:

• Line voltage regulation 0.1% current variation for 5 volt line change
• Load regulation 0.1% current variation for 50% change in load impedance
• Stability after warm-up 0.2%

Igniter:

• High voltage (30 KV) start pulse
• Highly shielded starter to prevent RF interference to computer and peripherals
• Continuous excitation correction monitors excitation beam
• Automatically corrects for short- or long-term fluctuations in intensity
• Located in sample compartment

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Excitation Control

• QuadraScopic™ monochromator:
• Two entrance and two exit ports for additional excitation sources and/or detectors
• Auto-calibrated and computer-controlled for scanning and positioning
• Continuously adjustable spectral bandwidth from 0 to 25 nm via micrometer slits
• Optional computer-controlled (patent pending) slits or micrometer slits
• Throughput 60% at 300 nm
• Stray light <0.02% two bandwidths from 365 nm Hg line
• Czerny-Turner optical configuration
• f/4 aperture ratio 200 mm focal length
• Reciprocal linear dispersion (with standard 1,200 l/mm grating) 4 nm/mm
• Accuracy ±0.5 nm
• Resolution 0.2 nm

Other monochromator options:

• Dual gratings to efficiently cover an extended wavelength range
• Double monochromators for lower stray light
• (useful for highly scattering samples such as suspensions or solids)

Grating:

• Standard ruled 1,200 l/mm blazed at 300 nm
• Optional holographic gratings available to cover the entire wavelength range from 180 nm to 2,000 nm

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QuadraCentric™ sample compartment:

• Four ports to accommodate various light sources and detectors
• Configurable in L-, T- or X-formats
• Quartz optics for optimum sample illumination and emission signal collection
• Roomy compartment permits easy access and accommodates various options
• Thermostatable 10 x 10 mm cuvette holder (requires external water bath, not included)
• One 10 x 10 mm quartz cuvette included
• Variable speed sample stirrer
• Lid-activated emission shutter to protect sensitive detectors
• Excitation and emission filter holders accommodate 2 in. square or 1 in. diameter round filters
• Provision for injection into sample
• Gas purgeable compartment

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Emission Control

• QuadraScopic™ monochromator, same as above
• Grating: Standard ruled 1,200 l/mm blazed at 400 nm
• Optional holographic gratings available to cover the entire wavelength range from 180 nm to 2,000 nm

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Detector

• Photomultiplier tube detector with wavelength range from 185 to 680 nm
• Extended wavelength range option to 900 nm available
• Analog or photon counting detection options, directly selectable on the photomultiplier housing.
• Analog for high signal levels or photon counting for low signal levels
• High voltage range 200 to 1,100 VDC, manually adjustable
• LCD display on detector indicates actual cathode voltage
• Temperature coefficient ±0.01% max. (+5 to 40 deg. C)
• Drift ±0.03%/hr. max. (after 15-minute warm-up)

Photon counting mode:

• Maximum count rate 3.8 MHz
• Pulse pair resolution 265 ns
• Rise time 20 ns
• Fall time 100 ns
• Pulse width 220 ns

Analog mode:

• Inherent noise 50 pA p-p typically (0.05 millisecond time constant)
• Offset correction ±50 nA.
• Gain settings 1, 0.1, 0.01, 0.001 x mA equals 1 volt
• Time constant settings 0.05, 0.5, 50 or 500 milliseconds
• LCD display on detector can be switched to display analog signal level directly
• Optional PMT available for detection between 700 to 900 nm
• Optional cooled PMT housing available for IR detection noise reduction

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Software and Electronics
FeliX32™ Advanced Fluorescence Analysis Software Package included a32-bit fluorescence analysis software developed specifically for fluorescence by PTI. Very easy to use Windows-based interface. Data exportable in many formats for use in other applications.

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Data Acquisition Characteristics

Signal to noise ratio of water Raman band 10,000:1

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Optional

• Optional special light sources such as lasers for specialized applications available
• Data rate 1000 points per seconds to 1 point per 200 seconds.

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Other

CE approved

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