Prepare for a revolution in High Definition Digital Imaging with PTI’s new ImHD Imaging platform for 2011. Imagine resolving the fine detail structure on Axons and Dendrites along with the cell body and not saturate your camera detection system. This is now possible with a camera dynamic range of 22,000:1. How about streaming 30–100 frames per second to disk at 5.5 megapixels per Image? That is a resolution of 2560 x 2160 pixels. This is 2.5 times higher resolution than Full HD television!
Calcium Measurement
- Intracellular free Ca2+
- Mitochondrial Ca2+
- Endoplasmic reticulum (ER) Ca2+ pool
- Lysosomal Ca2+
- Extracellular near membrane Ca2+
|
Other Ions
- Intracellular Mg2+
- Intracellular Mn2+
- Intracellular Zn2+
- Intracellular Sr2+
- Intracellular Na+
- Intracellular Cl-
- Cytosolic labile iron pool (LIP)
- Iron homeostasis
|
Membrane Potential
- Plasma membrane Potential
- Mitochondrial Membrane Potential
|
pH
- Intracellular pH
- Vacuolar pH
- Lysosomal pH
|
GFP & Variants
- Identifying cells coexpressing GFP for analysis
- Use mitoGFP to visualize mitochondria and measure mitochondrial calcium [Ca2+]m
- Use ratiometric GFP (redox-GFP) to measure cellular/mitochondria reduce/oxidize reaction
- HEK-293 cells transfected with the CaSR-pEGFP vector
- ECFP
- EYFP
|
FRET
- Donor Excitation Spectroscopy
- Emission intensity
- Donor emission intensity
- Acceptor emission intensity
- Ratio of donor/acceptor emission intensity
- Measure molecular proximity
- Binding of enzyme to itssubstrate
- Measure Endoplasmic reticulum (ER) Ca2+
|
Other Measurements
- Calcium imaging of
Single HEK 293 cell
- Taste Bud
- Pea Pollen tubes
- Respiratory burst & reactive oxygen species (ROS)
- NAD(P)H
- Changes in CaM availability
- Demonstration of selective plasma membrane permeabilization by digitonin in HEK293 cells
- Detection by immunofluorescence of PfCRT expression in HEK293 cells
- Receptor-mediated Glutamate release (by following NADH signal)
- Fura-dextra
- Cellular GSH level
- FL-aldosterone efflux
- Latency of response to exogenous ATP or ADP.
- Membrane traffic
- Liposome
- ER membrane
- Cell contractile activity & cell Size
- Cell Volume Measurement
- Phagocytosis
- Cell injury or death by PI fluorescent
- Metal induced cytotoxity
|
Calcium-related Measurement
- Ca2+ Waves
- Ca2+ wave quantitation
- Ca2+ wave induced bymechanical stimulation
- Ca2+ wave induced by mechanical wounding
- Ca2+ wave propagation
- In non-contacting cells
- Over a physical gap
- Ca2+ Oscillation induced by:
- Hg2+
- K+
- Heat
- ATP
- Dopamine
- FSH
- Caffine
- Ca2+ mobilization
- Ca2+ homeostasis
- Propagated cytoplasmic Ca2+cyt responses along the DVR wall.
- Simultaneous Measurement of intracellular Ca2+ and:
- Cell volume
- Phygocytosis
- Cl-
- Oxidase activation
- Ca2+ -CaM
- And Many More!
|
Non Ratiometric
Application |
Dye |
Chloride |
SPQ SPA
MQAE |
|
|
