Applications
Whether you’re working in biology, medicine, physical or material science, measuring proteins, DNA, FRET and others, the QuantaMaster™ 40 spectrofluorometer is the instrument for you! Samples are expensive but the sensitivity of the QuantaMaster™ 40 allows you use much less and measure more accurately and quickly than ever before. Myriad accessories and system enhancements makes the QuantaMaster™ 40 the perfect system for any application in fluorescence spectroscopy.
Below are some examples:
Protein Folding/Unfolding
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| Bovine serum albumin (BSA) unfolding monitored with PTI QuantaMaster™ 40 spectrofluorimeter equipped with the rapid temperature control accessory. Automated (macro) acquisition of BSA fluorescence spectra as a function of temperature showing the effect of thermal protein unfolding. |
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Fluorescence Anisotropy
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| Fluorescence anisotropy of bovine serum albumin (BSA) in PBS (pH=7.4) while ramping temperature with a QuantaMaster™ 40 equipped with dual emission channels and a rapid temperature change Peltier option. |
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Fluorescence Resonance Energy Transfer (FRET)
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| Titration monitored by FRET between Alexa-BSA complex and a Bodipy-labeled fatty acid. |
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Bioluminescence and Chemiluminescence
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| The unsurpassed sensitivity of the QuantaMaster™ 40 detection makes it a very capable instrument for measuring extremely weak chemiluminescence emission as illustrated by the cytochrome C/ hydrogen peroxide experiment. |
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Semiconductor Research
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| Due to its dedicated accessories such as a well designed solid sample holder and excellent stray light rejection characteristics, the QuantaMaster™ 40 is an excellent choice for the semiconductors research. Here, clean spectra from strongly scattering ZnO samples were measured with the QuantaMaster™ 40 equipped with a double excitation monochromator. |
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Electroluminescence and Photovoltaic Measurements
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| The flexibility of the modular design makes it easy to utilize the QuantaMaster™ 40 for more specialized applications, such as electroluminescence or photovoltaic measurement. Here, the figure shows an electrical response of a photovoltaic cell illuminated with the QuantaMaster™ 40 excitation monochromator equipped with an NIR grating. |
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| Fluorescence spectra from a strongly scattering paper sample measured at several excitation wavelengths. System: QuantaMaster™ 40 with optional solid sample holder. The solid sample accessory allows precise alignment of the sample in the light beam with the use of rotation and translation adjustments (sample courtesy of Cameron Thomson, Institute of Paper Science and Technology, Georgia Institute of Technology). |
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